Article
Binding of host factors to stabilized HIV-1 capsid tubes.
Virology - 1 Oct 2018
Selyutina Anastasia, Bulnes-Ramos Angel, Diaz-Griffero Felipe
Abstract excerpt
The capsid-binding assay is an in vitro experiment used to determine whether cellular proteins interact with the HIV-1 core. In vitro assembled HIV-1 capsids recapitulate the surface of the HIV-1 core. The assay involves the incubation of in vitro assembled HIV-1 capsid-nucleocapsid (CA-NC) complexes with the protein in question. Subsequently, the mixture is spun through a sucrose cushion using an...
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