A CRISPR‐Cpf1‐Assisted Non‐Homologous End Joining Genome Editing System of Mycobacterium smegmatis

Abstract excerpt

Mycobacterium smegmatis is an important model strain of Mycobacterium for scientific study because it is non-pathogenic and grows rapidly. However, research is limited by the low efficiency and time-consuming nature of existing genome editing tools. Although the Streptococcus pyogenes CRISPR-Cas9 system is widely used in bacterial genome editing, it cannot be introduced into M. smegmatis because of its toxicity....

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