Article
Electrochemical primer extension based on polyoxometalate electroactive labels for multiplexed detection of single nucleotide polymorphisms.
Biosensors & bioelectronics - 15 Oct 2018
Chahin Nassif, Uribe Laura A, Debela Ahmed M, Thorimbert Serge, Hasenknopf Bernold, Ortiz Mayreli, Katakis Ioannis, O'Sullivan Ciara K
Abstract excerpt
Polyoxymetalates (POMs) ([SiW11O39{Sn(CH2)2CO)}]4- and [P2W17O61{Sn(CH2)2CO)}]6-) were used to modify dideoxynucleotides (ddNTPs) through amide bond formation, and applied to the multiplexed detection of single nucleotide polymorphisms (SNPs) in an electrochemical primer extension reaction. Each gold electrode of an array was functionalised with a short single stranded thiolated DNA probe, specifically designed...
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