Article
Solid-phase recombinase polymerase amplification using ferrocene-labelled dNTPs for electrochemical detection of single nucleotide polymorphisms.
Biosensors & bioelectronics - 15 Feb 2022
Ortiz Mayreli, Jauset-Rubio Miriam, Kodr David, Simonova Anna, Hocek Michal, O'Sullivan Ciara K
Abstract excerpt
Hypertrophic cardiomyopathies (HCM) are the principal cause of sudden cardiac death in young athletes and it is estimated that 1 in 500 people have HCM. The aim of this work was to develop an electrochemical platform for the detection of HCM-associated SNP in the Myosin Heavy Chain 7 (MYH7) gene, in fingerprick blood samples. The platform exploits isothermal solid-phase primer elongation using recombinase...
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