Article
"Site and Mutation"-Specific Predictions Enable Minimal Directed Evolution Libraries.
ACS synthetic biology - 20 Jul 2018
Moore Jeffrey C, Rodriguez-Granillo Agustina, Crespo Alejandro, Govindarajan Sridhar, Welch Mark, Hiraga Kaori, Lexa Katrina, Marshall Nicholas, Truppo Matthew D
Abstract excerpt
Directed evolution experiments designed to improve the activity of a biocatalyst have increased in sophistication from the early days of completely random mutagenesis. Sequence-based and structure-based methods have been developed to identify "hotspot" positions that when randomized provide a higher frequency of beneficial mutations that improve activity. These focused mutagenesis methods reduce library sizes and...
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