Article
Directed Computational Evolution of Quorum-Quenching Lactonases from the Amidohydrolase Superfamily.
Structure (London, England : 1993) - 2 Jun 2020
Go Maybelle Kho, Zhao Li Na, Xue Bo, Supekar Shreyas, Robinson Robert C, Fan Hao, Yew Wen Shan
Abstract excerpt
In this work, we present a generalizable directed computational evolution protocol to effectively reduce the sequence space to be explored in rational enzyme design. The protocol involves in silico mutation modeling and substrate docking to rapidly identify mutagenesis hotspots that may enhance an enzyme's substrate binding and overall catalysis. By applying this protocol to a quorum-quenching Geobacillus...
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