Article
High-level production of single chain monellin mutants with enhanced sweetness and stability in tobacco chloroplasts.
Planta - 1 Aug 2018
Castiglia Daniela, Leone Serena, Tamburino Rachele, Sannino Lorenza, Fonderico Jole, Melchiorre Chiara, Carpentieri Andrea, Grillo Stefania, Picone Delia, Scotti Nunzia
Abstract excerpt
MAIN CONCLUSION: Plastid-based MNEI protein mutants retain the structure, stability and sweetness of their bacterial counterparts, confirming the attractiveness of the plastid transformation technology for high-yield production of recombinant proteins. The prevalence of obesity and diabetes has dramatically increased the industrial demand for the development and use of alternatives to sugar and traditional...
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