Article
Expression, purification and characterization of a novel double-sites mutant of the single-chain sweet-tasting protein monellin (MNEI) with both improved sweetness and stability.
Protein expression and purification - 1 Mar 2018
Zheng Weiwei, Yang Liu, Cai Chenggu, Ni Jinfeng, Liu Bo
Abstract excerpt
The sweet protein monellin has high sweet potency with limited stability. In this study, 3 double-sites mutants (E2N/E23A, E2N/Y65R and E23A/Y65R) of the single-chain monellin (MNEI) were constructed. The proteins were expressed in E. coli BL21 and purified to homogeneity by nickel affinity chromatography with yields above 10 mg/L cell culture. Introduction of a sweeter mutant E2N into E23A or Y65R (E2N/E23A and...
Topics
- Escherichia coli
- Hot Temperature
- Mutation
- Plant Proteins
- Protein Stability
- Recombinant Proteins
- Sweetening Agents