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Article

Expression of a single-chain monellin (MNEI) mutant with enhanced stability in transgenic mice milk.

Transgenic research - 1 Aug 2024

Lu Rui, Li Xiaoming, Hu Jian, Wang Yancui, Jin Le

Abstract excerpt

Monellin is a sweet protein that may be used as a safe and healthy sweetener. However, due to its low stability, the application of monellin is currently very limited. Here, we describe a wild-type, a double-sites mutant (E2N/E23A) and a triple-sites mutant (N14A/E23Q/S76Y) of single-chain monellin (MNEI) expressed in transgenic mice milk. Based on enzyme-linked immunoassay (ELISA), Western blot, and sweetness...

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Identifiers

PMID: 38858256
DOI: 10.1007/s11248-024-00389-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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