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Article

Protein stabilization with retained function of monellin using a split GFP system.

Scientific reports - 24 Aug 2018

Weiffert Tanja, Linse Sara

Abstract excerpt

Sweet proteins are an unexploited resource in the search for non-carbohydrate sweeteners mainly due to their low stability towards heating. Variants of the sweet protein monellin, with increased stability, were derived by an in vivo screening method based on the thermodynamic linkage between fragment complementation and protein stability. This approach depends on the correlation between mutational effects on the...

Topics

Amino Acid Sequence
Fluorescence
Green Fluorescent Proteins
Humans
Models, Molecular
Mutant Proteins
Mutation
Plant Proteins
Protein Denaturation
Protein Stability
Protein Structure, Secondary

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Taste

Temperature

Terminology as Topic

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Identifiers

PMID: 30143736
DOI: 10.1038/s41598-018-31177-z
PMCID: PMC6109104

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