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Article

Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Pietro Di Sansebastiano Gian, Fabrizio Barozzi

Abstract excerpt

Despite a long case history, the use of protoplasts in cell biology research still divides scientists but their weaknesses can be exploited as strengths. Transient expression in protoplasts can saturate protein-protein interactions very efficiently, inhibiting the process of interest more efficiently than other approaches at gene expression level. The method described here consists of an assay providing a...

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PMID: 28861831
DOI: 10.1007/978-1-4939-7262-3_19

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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