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Article

ChIP-exo interrogation of Crp, DNA, and RNAP holoenzyme interactions.

PloS one - 1 Jan 2018

Latif Haythem, Federowicz Stephen, Ebrahim Ali, Tarasova Janna, Szubin Richard, Utrilla Jose, Zengler Karsten, Palsson Bernhard O

Abstract excerpt

Numerous in vitro studies have yielded a refined picture of the structural and molecular associations between Cyclic-AMP receptor protein (Crp), the DNA motif, and RNA polymerase (RNAP) holoenzyme. In this study, high-resolution ChIP-exonuclease (ChIP-exo) was applied to study Crp binding in vivo and at genome-scale. Surprisingly, Crp was found to provide little to no protection of the DNA motif under activating...

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Identifiers

PMID: 29771928
DOI: 10.1371/journal.pone.0197272
PMCID: PMC5957442

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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