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Article

Detection of regulatory polymorphisms: high-throughput capillary DNase I footprinting.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Hancock Matthew, Shephard Elizabeth A

Abstract excerpt

We describe a method for high-throughput analysis of protein-binding sites in DNA using 96-well plates and capillary electrophoresis. The genomic DNA or plasmid DNA to be analyzed is amplified using fluorescent primers, incubated with an appropriate nuclear extract and treated with DNase I. Separation of the DNase I-generated fragments and co-analysis of their base sequences identify the position of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23475685
DOI: 10.1007/978-1-62703-321-3_23

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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