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Article

A Follow-Up of the Multicenter Collaborative Study on HIV-1 Drug Resistance and Tropism Testing Using 454 Ultra Deep Pyrosequencing.

PloS one - 1 Jan 2016

St John Elizabeth P, Simen Birgitte B, Turenchalk Gregory S, Braverman Michael S, Abbate Isabella, Aerssens Jeroen, Bouchez Olivier, Gabriel Christian, Izopet Jacques, Meixenberger Karolin, Di Giallonardo Francesca, Schlapbach Ralph, Paredes Roger, Sakwa James, Schmitz-Agheguian Gudrun G, Thielen Alexander, Victor Martin, Metzner Karin J, Däumer Martin P

Abstract excerpt

BACKGROUND: Ultra deep sequencing is of increasing use not only in research but also in diagnostics. For implementation of ultra deep sequencing assays in clinical laboratories for routine diagnostics, intra- and inter-laboratory testing are of the utmost importance. METHODS: A multicenter study was conducted to validate an updated assay design for 454 Life Sciences' GS FLX Titanium system targeting...

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Identifiers

PMID: 26756901
DOI: 10.1371/journal.pone.0146687
PMCID: PMC4710461

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