Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.

doi:10.1073/pnas.1803564115

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Article

Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.

Proceedings of the National Academy of Sciences of the United States of America - 22 May 2018

Xu Xingya, Kanai Ryuta, Nakazawa Norihiko, Wang Li, Toyoshima Chikashi, Yanagida Mitsuhiro

Abstract excerpt

Cohesin is a fundamental protein complex that holds sister chromatids together. Separase protease cleaves a cohesin subunit Rad21/SCC1, causing the release of cohesin from DNA to allow chromosome segregation. To understand the functional organization of cohesin, we employed next-generation whole-genome sequencing and identified numerous extragenic suppressors that overcome either inactive separase/Cut1 or...

Topics

Cell Cycle Proteins
Chromatids
Chromosomal Proteins, Non-Histone
Chromosome Segregation
DNA, Fungal
Models, Molecular
Mutation
Nuclear Proteins
Phosphoproteins
Phosphorylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Protein Subunits

Schizosaccharomyces

Schizosaccharomyces pombe Proteins

Suppression, Genetic

Cohesins

Source

Open in PubMed Open full text

Identifiers

PMID: 29735656
DOI: 10.1073/pnas.1803564115
PMCID: PMC6003501

Genes

SMC3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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