Article
Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.
Stem cell research - 1 May 2018
Xu An, Zhou Ruoji, Tu Jian, Huo Zijun, Zhu Dandan, Wang Donghui, Gingold Julian A, Mata Helen, Rao Pulivarthi H, Liu Mo, Mohamed Alaa M T, Kong Celine Shuet Lin, Jewell Brittany E, Xia Weiya, Zhao Ruiying, Hung Mien-Chie, Lee Dung-Fang
Abstract excerpt
Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.
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