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A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing.

Stem cell research - 1 Mar 2018

Zhou Ruoji, Xu An, Wang Donghui, Zhu Dandan, Mata Helen, Huo Zijun, Tu Jian, Liu Mo, Mohamed Alaa M T, Jewell Brittany E, Gingold Julian, Xia Weiya, Rao Pulivarthi H, Hung Mien-Chie, Zhao Ruiying, Lee Dung-Fang

Abstract excerpt

The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal...

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PMID: 29414603
DOI: 10.1016/j.scr.2018.01.035

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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