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Defining essential genes for human pluripotent stem cells by CRISPR-Cas9 screening in haploid cells.

Nature cell biology - 1 May 2018

Yilmaz Atilgan, Peretz Mordecai, Aharony Aviram, Sagi Ido, Benvenisty Nissim

Abstract excerpt

The maintenance of pluripotency requires coordinated expression of a set of essential genes. Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. With this we could allude to an intrinsic bias of essentiality across cellular compartments, uncover two opposing...

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PMID: 29662178
DOI: 10.1038/s41556-018-0088-1

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