Article
A new hybrid approach for MHC genotyping: high-throughput NGS and long read MinION nanopore sequencing, with application to the non-model vertebrate Alpine chamois (Rupicapra rupicapra).
Heredity - 1 Oct 2018
Fuselli S, Baptista R P, Panziera A, Magi A, Guglielmi S, Tonin R, Benazzo A, Bauzer L G, Mazzoni C J, Bertorelle G
Abstract excerpt
The major histocompatibility complex (MHC) acts as an interface between the immune system and infectious diseases. Accurate characterization and genotyping of the extremely variable MHC loci are challenging especially without a reference sequence. We designed a combination of long-range PCR, Illumina short-reads, and Oxford Nanopore MinION long-reads approaches to capture the genetic variation of the MHC II DRB...
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