Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Large-scale genotyping of highly polymorphic loci by next-generation sequencing: how to overcome the challenges to reliably genotype individuals?

Heredity - 1 May 2015

Ferrandiz-Rovira M, Bigot T, Allainé D, Callait-Cardinal M-P, Cohas A

Abstract excerpt

Studying the different roles of adaptive genes is still a challenge in evolutionary ecology and requires reliable genotyping of large numbers of individuals. Next-generation sequencing (NGS) techniques enable such large-scale sequencing, but stringent data processing is required. Here, we develop an easy to use methodology to process amplicon-based NGS data and we apply this methodology to reliably genotype four...

Topics

Join the communities discussing this publication.

Alleles
Genes, MHC Class I
Genes, MHC Class II
Genotyping Techniques
High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microsatellite Repeats

Reproducibility of Results

Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 25757407
DOI: 10.1038/hdy.2015.13
PMCID: PMC4815514

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Large-scale genotyping of highly polymorphic loci by next-generation sequencing: how to overcome the challenges to reliably genotype individuals?PMID 25757407Read ↗

MHC genotyping of non-model organisms using next-generation sequencing: a new methodology to deal with artefacts and allelic dropout.Semantic proximity Read ↗

Next-generation genotyping of hypervariable loci in many individuals of a non-model species: technical and theoretical implications.Semantic proximity Read ↗

A robust sequencing assay of a thousand amplicons for the high-throughput population monitoring of Alpine ibex immunogeneticsSemantic proximity Read ↗

Genotyping-by-sequencing (GBS): a novel, efficient and cost-effective genotyping method for cattle using next-generation sequencing.Semantic proximity Read ↗

Coverage recommendation for genotyping analysis of highly heterologous species using next-generation sequencing technology.Semantic proximity Read ↗

PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches.Semantic proximity Read ↗

Development and evaluation of a rapid and cost-efficient NGS-based MHC class I genotyping method for macaques by using a prevalent short-read sequencer.Semantic proximity Read ↗

Complete Genomic Assembly of Mauritian Cynomolgus Macaque Killer Ig-like Receptor and Natural Killer Group 2 Haplotypes.Semantic proximity Read ↗

Massive parallel MHC genotyping: titanium that shines.Semantic proximity Read ↗

Template-specific optimization of NGS genotyping pipelines reveals allele-specific variation in MHC gene expression.Semantic proximity Read ↗

Ultra-deep Illumina sequencing accurately identifies MHC class IIb alleles and provides evidence for copy number variation in the guppy (Poecilia reticulata).Semantic proximity Read ↗

A novel workflow to improve genotyping of multigene families in wildlife species: An experimental set-up with a known model system.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.