Article
Ku DNA End-Binding Activity Promotes Repair Fidelity and Influences End-Processing During Nonhomologous End-Joining in Saccharomyces cerevisiae.
Genetics - 1 May 2018
Emerson Charlene H, Lopez Christopher R, Ribes-Zamora Albert, Polleys Erica J, Williams Christopher L, Yeo Lythou, Zaneveld Jacques E, Chen Rui, Bertuch Alison A
Abstract excerpt
The Ku heterodimer acts centrally in nonhomologous end-joining (NHEJ) of DNA double-strand breaks (DSB). Saccharomyces cerevisiae Ku, like mammalian Ku, binds and recruits NHEJ factors to DSB ends. Consequently, NHEJ is virtually absent in yeast Ku null (yku70∆ or yku80∆) strains. Previously, we unexpectedly observed imprecise NHEJ proficiency in a yeast Ku mutant with impaired DNA end-binding (DEB). However, how...
Topics
- DNA End-Joining Repair
- DNA Repair
- DNA-Binding Proteins
- Ku Autoantigen
- Mutation
- Protein Binding
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Telomere