Base-pairing between 23S rRNA and tRNA in the ribosomal A site.

doi:10.1016/s1097-2765(00)80395-0

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Article

Base-pairing between 23S rRNA and tRNA in the ribosomal A site.

Molecular cell - 1 Nov 1999

Kim D F, Green R

Abstract excerpt

The aminoacyl (A site) tRNA analog 4-thio-dT-p-C-p-puromycin (s4TCPm) photochemically cross-links with high efficiency and specificity to G2553 of 23S rRNA and is peptidyl transferase reactive in its cross-linked state, establishing proximity between the highly conserved 2555 loop in domain V of 23S rRNA and the universally conserved CCA end of tRNA. To test for base-pairing interactions between 23S rRNA and...

Topics

2-Aminopurine
Base Pairing
Binding Sites
Conserved Sequence
Escherichia coli
Genes, Bacterial
Geobacillus stearothermophilus
Hydrogen Bonding
Kinetics
Mutagenesis, Site-Directed
Mutation
Nucleic Acid Conformation
Peptidyl Transferases
Puromycin
RNA, Ribosomal, 23S
RNA, Transfer, Amino Acyl
Ribosomes
Suppression, Genetic

Source

Open in PubMed

Identifiers

PMID: 10619032
DOI: 10.1016/s1097-2765(00)80395-0

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