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Article

Bioluminescence and kinetic aspects of double mutated aequorin variants.

International journal of biological macromolecules - 1 Jun 2018

Zeinoddini Mehdi, Fathi-Roudsari Mehrnoosh, Hosseinkhani Saman, Khajeh Khosro

Abstract excerpt

Aequorin as an old small calcium-sensitive photoprotein is a blue fluorescence protein which converts coelenterazine (a substrate) to coelenteramide with a flash type emission. The decay kinetics and emission properties of this protein can be changed using directed mutagenesis of crucial amino acid residue. In this work, we prepared three double mutants: Y82F/W86F, Y82F/D153G, and W86F/D153G. According to our...

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Source

Identifiers

PMID: 29382580
DOI: 10.1016/j.ijbiomac.2018.01.170

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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