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Article

Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein.

FEBS letters - 14 Feb 2005

Stepanyuk Galina A, Golz Stefan, Markova Svetlana V, Frank Ludmila A, Lee John, Vysotski Eugene S

Abstract excerpt

The bioluminescence spectra from the Ca2+-regulated photoproteins aequorin (lambdamax=469 nm) and obelin (lambdamax=482 nm) differ because aequorin has an H-bond from its Tyr82 to the bound coelenteramide, not present in obelin at the corresponding Phe88. Substitutions of this Phe88 by Tyr, Trp, or His shifted the obelin bioluminescence to shorter wavelength with F88Y having lambdamax=453 nm. Removal of the...

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PMID: 15710383
DOI: 10.1016/j.febslet.2005.01.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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