Article
Requirement of the C-terminal proline residue for stability of the Ca(2+)-activated photoprotein aequorin.
The Biochemical journal - 1 Jul 1993
Watkins N J, Campbell A K
Abstract excerpt
cDNA coding for the Ca(2+)-activated photoprotein aequorin from the jellyfish Aequorea victoria has been engineered to investigate the role of the C-terminal proline residue in bioluminescence. Recombinant aequorin proteins were synthesized by PCR followed by in vitro transcription/translation, and characterized by specific activity, stability, and affinity for coelenterazine. The C-terminal proline residue of...
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