Requirement of the C-terminal proline residue for stability of the Ca(2+)-activated photoprotein aequorin.

doi:10.1042/bj2930181

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Article

Requirement of the C-terminal proline residue for stability of the Ca(2+)-activated photoprotein aequorin.

The Biochemical journal - 1 Jul 1993

Watkins N J, Campbell A K

Abstract excerpt

cDNA coding for the Ca(2+)-activated photoprotein aequorin from the jellyfish Aequorea victoria has been engineered to investigate the role of the C-terminal proline residue in bioluminescence. Recombinant aequorin proteins were synthesized by PCR followed by in vitro transcription/translation, a...

Topics

Aequorin
Animals
Base Sequence
Calcium
DNA
Electrophoresis, Agar Gel
Glutamates
Glutamic Acid
Histidine
Imidazoles
In Vitro Techniques
Luminescent Measurements
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Proline
Protein Biosynthesis
Pyrazines

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