Article
Hydrogen-bond networks between the C-terminus and Arg from the first α-helix stabilize photoprotein molecules.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Mar 2014
Eremeeva Elena V, Burakova Ludmila P, Krasitskaya Vasilisa V, Kudryavtsev Alexander N, Shimomura Osamu, Frank Ludmila A
Abstract excerpt
Previous studies have stated that aequorin loses most of its bioluminescence activity upon modification of the C-terminus, thus limiting the production of photoprotein fusion proteins at its N-terminus. In the present work, we investigate the importance of the C-terminal proline and the hydrogen bonds it forms for photoprotein active complex formation, stability and functional activity. According to the crystal...
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