Article
RedquorinXS Mutants with Enhanced Calcium Sensitivity and Bioluminescence Output Efficiently Report Cellular and Neuronal Network Activities.
International journal of molecular sciences - 22 Oct 2020
Bakayan Adil, Picaud Sandrine, Malikova Natalia P, Tricoire Ludovic, Lambolez Bertrand, Vysotski Eugene S, Peyriéras Nadine
Abstract excerpt
Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+-dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP-aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening...
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