Article
APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks.
Nature structural & molecular biology - 1 Jan 2018
Lei Liqun, Chen Hongquan, Xue Wei, Yang Bei, Hu Bian, Wei Jia, Wang Lijie, Cui Yiqiang, Li Wei, Wang Jianying, Yan Lei, Shang Wanjing, Gao Jimin, Sha Jiahao, Zhuang Min, Huang Xingxu, Shen Bin, Yang Li, Chen Jia
Abstract excerpt
The APOBEC-AID family of cytidine deaminase prefers single-stranded nucleic acids for cytidine-to-uridine deamination. Single-stranded nucleic acids are commonly involved in the DNA repair system for breaks generated by CRISPR-Cas9. Here, we show in human cells that APOBEC3 can trigger cytidine deamination of single-stranded oligodeoxynucleotides, which ultimately results in base substitution mutations in genomic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.