Article
In vivo dynamics of enterovirus protease revealed by fluorescence resonance emission transfer (FRET) based on a novel FRET pair.
Biochemical and biophysical research communications - 23 Feb 2007
Hsu Yueh-Ying, Liu Yu-Ning, Wang Wenyen, Kao Fu-Jen, Kung Szu-Hao
Abstract excerpt
An in vivo protease assay suitable for analysis by fluorescence resonance energy transfer (FRET) was developed on the basis of a novel FRET pair. The specifically designed fusion substrate consists of green fluorescent protein 2 (GFP2)-peptide-red fluorescent protein 2 (DsRed2), with a cleavage motif for the enterovirus 2A protease (2Apro) embedded within the peptide region. FRET can be readily visualized in...
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