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Article

Unintended Side Effects of Transformation Are Very Rare in Cryptococcus neoformans.

G3 (Bethesda, Md.) - 2 Mar 2018

Friedman Ryan Z, Gish Stacey R, Brown Holly, Brier Lindsey, Howard Nicole, Doering Tamara L, Brent Michael R

Abstract excerpt

Received wisdom in the field of fungal biology holds that the process of editing a genome by transformation and homologous recombination is inherently mutagenic. However, that belief is based on circumstantial evidence. We provide the first direct measurement of the effects of transformation on a fungal genome by sequencing the genomes of 29 transformants and 30 untransformed controls with high coverage. Contrary...

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Share to Cryptococcus neoformans
Share to DNA Copy Number Variations

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Fungal

Share to Genome, Fungal

Share to Mutagenesis, Insertional

Share to Reverse Genetics

Share to Sequence Deletion

Share to Transformation, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 29305388
DOI: 10.1534/g3.117.300357
PMCID: PMC5844303

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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