Article
A PCR-based strategy to generate integrative targeting alleles with large regions of homology.
Microbiology (Reading, England) - 1 Aug 2002
Davidson Robert C, Blankenship Jill R, Kraus Peter R, de Jesus Berrios Marisol, Hull Christina M, D'Souza Cletus, Wang Ping, Heitman Joseph
Abstract excerpt
Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle for which genetic and molecular techniques are well developed. The entire genome sequence of one C. neoformans strain is nearing completion. The efficient use of this sequence is dependent upon the development of methods to perform more rapid genetic analysis including gene-disruption techniques. A modified PCR overlap...
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