Article
Efficient identification of compounds suppressing protein precipitation via solvent screening using serial deletion mutants of the target protein.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Feb 2018
Yunoki Kaori, Yoshinaga Sosuke, Takeda Mitsuhiro, Nagano Ryohei, Tsuchiya Yusuke, Sonoda Akihiro, Tsuji Tatsuichiro, Hirakane Makoto, Toda Etsuko, Terashima Yuya, Matsushima Kouji, Terasawa Hiroaki
Abstract excerpt
The control of protein solubility is a subject of broad interest. Although several solvent screening methods are available to search for compounds that enhance protein solubilization, their performance is influenced by the intrinsic solubility of the tested protein. We now present a method for screening solubilizing compounds, using an array of N- or C-terminal deletion mutants of the protein. A key behind this...
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