Article
Structure-based substitutions for increased solubility of a designed protein.
Protein engineering - 1 Oct 2003
Mosavi Leila K, Peng Zheng-Yu
Abstract excerpt
Manipulation of protein solubility is important for many aspects of protein design and engineering. Previously, we designed a series of consensus ankyrin repeat proteins containing one, two, three and four identical repeats (1ANK, 2ANK, 3ANK and 4ANK). These proteins, particularly 4ANK, are intended for use as a universal scaffold on which specific binding sites can be constructed. Despite being well folded and...
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