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Article

Identifying protein construct variants with increased crystallization propensity--a case study.

Protein science : a publication of the Protein Society - 1 Dec 2006

Malawski Guido A, Hillig Roman C, Monteclaro Felipe, Eberspaecher Uwe, Schmitz Arndt A P, Crusius Kerstin, Huber Martina, Egner Ursula, Donner Peter, Müller-Tiemann Beate

Abstract excerpt

This study describes an efficient multiparallel automated workflow of cloning, expression, purification, and crystallization of a large set of construct variants for isolated protein domains aimed at structure determination by X-ray crystallography. This methodology is applied to MAPKAP kinase 2, a key enzyme in the inflammation pathway and thus an attractive drug target. The study reveals a distinct subset of...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 17132859
DOI: 10.1110/ps.062491906
PMCID: PMC2242438

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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