Article
Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2.
Cell reports - 12 Dec 2017
Kaniecki Kyle, De Tullio Luisina, Gibb Bryan, Kwon Youngho, Sung Patrick, Greene Eric C
Abstract excerpt
Srs2 is a superfamily 1 (SF1) helicase and antirecombinase that is required for genome integrity. However, the mechanisms that regulate Srs2 remain poorly understood. Here, we visualize Srs2 as it acts upon single-stranded DNA (ssDNA) bound by the Rad51 recombinase. We demonstrate that Srs2 is a processive translocase capable of stripping thousands of Rad51 molecules from ssDNA at a rate of ∼50 monomers/s. We...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.