Article
Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination.
Journal of immunology (Baltimore, Md. : 1950) - 15 Jan 2018
Amodio Donato, Cotugno Nicola, Macchiarulo Giulia, Rocca Salvatore, Dimopoulos Yiannis, Castrucci Maria Rita, De Vito Rita, Tucci Filippo M, McDermott Adrian B, Narpala Sandeep, Rossi Paolo, Koup Richard A, Palma Paolo, Petrovas Constantinos
Abstract excerpt
Generation of Ag-specific humoral responses requires the orchestrated development and function of highly specialized immune cells in secondary lymphoid organs. We used a multiparametric approach combining flow cytometry, confocal microscopy, and histocytometry to analyze, for the first time to our knowledge in children, tonsils from seasonal influenza-vaccinated children. We used these novel imaging assays to...
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