Article
Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis.
Scientific reports - 30 Nov 2017
Kaur Prameet, Saunders Timothy E, Tolwinski Nicholas S
Abstract excerpt
Optogenetics allows precise, fast and reversible intervention in biological processes. Light-sheet microscopy allows observation of the full course of Drosophila embryonic development from egg to larva. Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo. To develop this method, we investigated the regulation of...
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