Article
Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding.
Scientific reports - 14 Nov 2017
Zwolak Adam, Leettola Catherine N, Tam Susan H, Goulet Dennis R, Derebe Mehabaw G, Pardinas Jose R, Zheng Songmao, Decker Rose, Emmell Eva, Chiu Mark L
Abstract excerpt
Methods to rapidly generate high quality bispecific antibodies (BsAb) having normal half-lives are critical for therapeutic programs. Here, we identify 3 mutations (T307P, L309Q, and Q311R or "TLQ") in the Fc region of human IgG1 which disrupt interaction with protein A while enhancing interaction with FcRn. The mutations are shown to incrementally alter the pH at which a mAb elutes from protein A affinity resin....
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