Article
Modulation of protein A binding allows single-step purification of mouse bispecific antibodies that retain FcRn binding.
mAbs - 1 Jan 2000
Zwolak Adam, Armstrong Anthony A, Tam Susan H, Pardinas Jose R, Goulet Dennis R, Zheng Songmao, Brosnan Kerry, Emmell Eva, Luo Jeffrey, Gilliland Gary L, Chiu Mark L
Abstract excerpt
The increased number of bispecific antibodies (BsAb) under therapeutic development has resulted in a need for mouse surrogate BsAbs. Here, we describe a one-step method for generating highly pure mouse BsAbs suitable for in vitro and in vivo studies. We identify two mutations in the mouse IgG2a and IgG2b Fc region: one that eliminates protein A binding and one that enhances protein A binding by 8-fold. We show...
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