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Article

Semi-rational engineering of CYP153A35 to enhance ω-hydroxylation activity toward palmitic acid.

Applied microbiology and biotechnology - 1 Jan 2018

Jung Eunok, Park Beom Gi, Yoo Hee-Wang, Kim Joonwon, Choi Kwon-Young, Kim Byung-Gee

Abstract excerpt

CYP153A35 from Gordonia alkanivorans was recently characterized as fatty acid ω-hydroxylase. To enhance the catalytic activity of CYP153A35 toward palmitic acid, site-directed saturation mutagenesis was attempted using a semi-rational approach that combined structure-based computational analysis and subsequent saturation mutagenesis. Using colorimetric high-throughput screening (HTS) method based on...

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PMID: 29124283
DOI: 10.1007/s00253-017-8584-y

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