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Article

Semi-rational Engineering of a Promiscuous Fatty Acid Hydratase for Alteration of Regioselectivity.

Chembiochem : a European journal of chemical biology - 16 Feb 2022

Zhang Yan, Breum Niels Mikkel Dyrby, Schubert Sune, Hashemi Negin, Kyhnau Rikke, Knauf Marius Sandholt, Mathialakan Masuthan, Takeuchi Michiki, Kishino Shigenobu, Ogawa Jun, Kristensen Peter, Guo Zheng, Eser Bekir Engin

Abstract excerpt

Fatty acid hydratases (FAHs) catalyze regio- and stereo-selective hydration of unsaturated fatty acids to produce hydroxy fatty acids. Fatty acid hydratase-1 (FA-HY1) from Lactobacillus Acidophilus is the most promiscuous and regiodiverse FAH identified so far. Here, we engineered binding site residues of FA-HY1 (S393, S395, S218 and P380) by semi-rational protein engineering to alter regioselectivity. Although...

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PMID: 34929055
DOI: 10.1002/cbic.202100606

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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