Article
Structural transitions in the transcription elongation complexes of bacterial RNA polymerase during σ-dependent pausing.
Nucleic acids research - 1 Apr 2012
Zhilina Ekaterina, Esyunina Daria, Brodolin Konstantin, Kulbachinskiy Andrey
Abstract excerpt
A transcription initiation factor, the σ(70) subunit of Escherichia coli RNA polymerase (RNAP) induces transcription pausing through the binding to a promoter-like pause-inducing sequence in the DNA template during transcription elongation. Here, we investigated the mechanism of σ-dependent pausing using reconstituted transcription elongation complexes which allowed highly efficient and precisely controlled pause...
Topics
- DNA
- DNA Footprinting
- DNA-Directed RNA Polymerases
- Escherichia coli Proteins
- Exodeoxyribonucleases
- Mutation
- Nucleic Acid Denaturation
- Oligonucleotides
- Potassium Permanganate
- RNA
- Sigma Factor