CRISPR-Cas9–mediated saturated mutagenesis screen predicts clinical drug resistance with improved accuracy

Abstract excerpt

Significance Many therapeutic strategies are hampered by the development of drug resistance. High-throughput random mutagenesis screens represent a promising approach for identifying mutations that lead to drug resistance, but have often identified more resistant mutations than are observed in patients, raising questions of their clinical significance. We developed an improved high-throughput mutagenesis...

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