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Article

Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands.

Nature biotechnology - 1 Oct 2021

Cohen Joshua D, Douville Christopher, Dudley Jonathan C, Mog Brian J, Popoli Maria, Ptak Janine, Dobbyn Lisa, Silliman Natalie, Schaefer Joy, Tie Jeanne, Gibbs Peter, Tomasetti Cristian, Papadopoulos Nickolas, Kinzler Kenneth W, Vogelstein Bert

Abstract excerpt

Identification and quantification of low-frequency mutations remain challenging despite improvements in the baseline error rate of next-generation sequencing technologies. Here, we describe a method, termed SaferSeqS, that addresses these challenges by (1) efficiently introducing identical molecular barcodes in the Watson and Crick strands of template molecules and (2) enriching target sequences with...

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Identifiers

PMID: 33941929
DOI: 10.1038/s41587-021-00900-z
PMCID: PMC8627329

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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