Enhanced Genome Editing Tools For Multi‐Gene Deletion Knock‐Out Approaches Using Paired CRISPR sgRNAs in CHO Cells

Abstract excerpt

Since the establishment of clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9, powerful strategies for engineering of CHO cell lines have emerged. Nevertheless, there is still room to expand the scope of the CRISPR tool box for further applications to improve CHO cell factories. Here, the authors demonstrate activity of the alternative CRISPR endonuclease Cpf1 in CHO-K1 for the first time and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to CAR-T cell therapy research
• Share to CRISPR and Genetic Engineering
• Share to Viral Infectious Diseases and Gene Expression in Insects