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Enhancing Protein Production Yield from Chinese Hamster Ovary Cells by CRISPR Interference

18 Apr 2017

Abstract excerpt

Chinese hamster ovary (CHO) cells are an important host for biopharmaceutical production. Generation of stable CHO cells typically requires cointegration of dhfr and a foreign gene into chromosomes and subsequent methotrexate (MTX) selection for coamplification of dhfr and foreign gene. CRISPR interference (CRISPRi) is an emerging system that effectively suppresses gene transcription through the coordination of...

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PMID: 28418635
DOI: 10.1021/acssynbio.7b00020

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