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Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

11 Apr 2019

Abstract excerpt

Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Use of these in next generation sequencing (NGS) is a priority. Nine formalin-fixed paraffin-embedded (FFPE) DNA extraction methods were evaluated using twelve FFPE samples of varying tissue types. Quality assessment included total yield, percent dsDNA, fragment analysis and...

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PMID: 30973937
DOI: 10.1371/journal.pone.0211400

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