Article
Fragment analysis represents a suitable approach for the detection of hotspot c.7541_7542delCT NOTCH1 mutation in chronic lymphocytic leukemia.
Leukemia research - 1 Sept 2017
Vavrova Eva, Kantorova Barbara, Vonkova Barbara, Kabathova Jitka, Skuhrova-Francova Hana, Diviskova Eva, Letocha Ondrej, Kotaskova Jana, Brychtova Yvona, Doubek Michael, Mayer Jiri, Pospisilova Sarka
Abstract excerpt
The hotspot c.7541_7542delCT NOTCH1 mutation has been proven to have a negative clinical impact in chronic lymphocytic leukemia (CLL). However, an optimal method for its detection has not yet been specified. The aim of our study was to examine the presence of the NOTCH1 mutation in CLL using three commonly used molecular methods. Sanger sequencing, fragment analysis and allele-specific PCR were compared in the...
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