Article
A Novel Assay for the Identification of NOTCH1 PEST Domain Mutations in Chronic Lymphocytic Leukemia.
BioMed research international - 1 Jan 2016
Campregher Paulo Vidal, Petroni Roberta Cardoso, Muto Nair Hideko, Sitnik Roberta, de Carvalho Flavia Pereira, Bacal Nydia Strachman, Velloso Elvira Deolinda Rodrigues Pereira, Oliveira Gislaine Borba, Pinho João Renato Rebello, Torres Davi Coe, Mansur Marcela Braga, Hassan Rocio, Lorand-Metze Irene Gyongyvér Heidemarie, Chiattone Carlos Sérgio, Hamerschlak Nelson, Mangueira Cristovão Luis Pitangueira
Abstract excerpt
Aims. To develop a fast and robust DNA-based assay to detect insertions and deletions mutations in exon 34 that encodes the PEST domain of NOTCH1 in order to evaluate patients with chronic lymphocytic leukemia (CLL). Methods. We designed a multiplexed allele-specific polymerase chain reaction (PCR) combined with a fragment analysis assay to detect specifically the mutation c.7544_7545delCT and possibly other...
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