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Article

Interpreting Reverse Transcriptase Termination and Mutation Events for Greater Insight into the Chemical Probing of RNA.

Biochemistry - 5 Sept 2017

Sexton Alec N, Wang Peter Y, Rutenberg-Schoenberg Michael, Simon Matthew D

Abstract excerpt

Chemical probing has the power to provide insight into RNA conformation in vivo and in vitro, but interpreting the results depends on methods to detect the chemically modified nucleotides. Traditionally, the presence of modified bases was inferred from their ability to halt reverse transcriptase during primer extension and the locations of termination sites observed by electrophoresis or sequencing. More...

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PMID: 28820243
DOI: 10.1021/acs.biochem.7b00323
PMCID: PMC5648349

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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